Construction of an acetylcholinesterase sensor based on synthesized paramagnetic nanoparticles, a simple tool for neurotoxic compounds assay

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dc.contributor.authorKostelník, Adamcs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorČegan, Alexandercs
dc.contributor.authorPohanka, Miroslavcs
dc.coverage.issue4cs
dc.coverage.volume17cs
dc.date.accessioned2020-08-04T11:03:41Z
dc.date.available2020-08-04T11:03:41Z
dc.date.issued2017-04-30cs
dc.description.abstractMagnetic particles (MPs) have been widely used in biological applications in recent years as a carrier for various molecules. Their big advantage is in repeated use of immobilized molecules including enzymes. Acetylcholinesterase (AChE) is an enzyme playing crucial role in neurotransmission and the enzyme is targeted by various molecules like Alzheimer’s drugs, pesticides and warfare agents. In this work, an electrochemical biosensor having AChE immobilized onto MPs and stabilized through glutaraldehyde (GA) molecule was proposed for assay of the neurotoxic compounds. The prepared nanoparticles were modified by pure AChE and they were used for the measurement anti-Alzheimer’s drug galantamine and carbamate pesticide carbofuran with limit of detection 1.5 µM and 20 nM, respectively. All measurements were carried out using screen-printed sensor with carbon working, silver reference, and carbon auxiliary electrode. Standard Ellman’s assay was used for validation measurement of both inhibitors. Part of this work was the elimination of reversible inhibitors represented by galantamine from the active site of AChE. For this purpose, we used a lower pH to get the original activity of AChE after inhibition by galantamine. We also observed decarbamylation of the AChE-carbofuran adduct. Influence of organic solvents to AChE as well as repeatability of measurement with MPs with AChE was also established.en
dc.formattextcs
dc.format.extent1-12cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2017, vol. 17, issue 4, p. 1-12.en
dc.identifier.doi10.3390/s17040676cs
dc.identifier.issn1424-8220cs
dc.identifier.other137740cs
dc.identifier.urihttp://hdl.handle.net/11012/137242
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofSENSORScs
dc.relation.urihttps://www.mdpi.com/1424-8220/17/4/676cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectacetylcholinesteraseen
dc.subjectmagnetic particlesen
dc.subjectelectrochemistryen
dc.subjectscreen-printed sensoren
dc.subjectcarbofuranen
dc.subjectgalantamineen
dc.subjectnanomaterialen
dc.subjectnanoparticlesen
dc.titleConstruction of an acetylcholinesterase sensor based on synthesized paramagnetic nanoparticles, a simple tool for neurotoxic compounds assayen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-137740en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:03:41en
sync.item.modts2020.08.04 12:25:13en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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