Plasmide DNA Isolation from Bacteria and Transfection to HEK293 Cell Line

Simple item page
but.event.date23.04.2015cs
but.event.titleStudent EEICT 2015cs
dc.contributor.authorKarmazínová, K.
dc.date.accessioned2015-08-25T08:42:51Z
dc.date.available2015-08-25T08:42:51Z
dc.date.issued2015cs
dc.description.abstractIsolation DNA is a one of the basic methods in molecular biology. There are several methods of DNA amplification and isolation. In this paper phenol-chloroform extraction of three plasmid types is used: Channelrhodopsin-2, ASAP and KIR. Seven plasmids were isolated in total. These plasmids are then validated using gel electrophoresis. Successfully isolated plasmids are then transfected to HEK293 and taken on confocal microscope 24 hours after transfection.en
dc.formattextcs
dc.format.extent125-127cs
dc.format.mimetypeapplication/pdfen
dc.identifier.citationProceedings of the 21st Conference STUDENT EEICT 2015. s. 125-127. ISBN 978-80-214-5148-3cs
dc.identifier.isbn978-80-214-5148-3
dc.identifier.urihttp://hdl.handle.net/11012/42951
dc.language.isocscs
dc.publisherVysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.relation.ispartofProceedings of the 21st Conference STUDENT EEICT 2015en
dc.relation.urihttp://www.feec.vutbr.cz/EEICT/cs
dc.rights© Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.rights.accessopenAccessen
dc.subjectDNA isolationen
dc.subjectcompetent cellsen
dc.subjectHEK293en
dc.subjecttransfectionen
dc.titlePlasmide DNA Isolation from Bacteria and Transfection to HEK293 Cell Lineen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
eprints.affiliatedInstitution.departmentFakulta elektrotechniky a komunikačních technologiícs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
eeict2015-125-karmazinova.pdf
Size:
957.26 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Student EEICT 2015