Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

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dc.contributor.authorMoulick, Amitavacs
dc.contributor.authorMilosavljević, Vedrancs
dc.contributor.authorVlachová, Janacs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorPodgajny, Robertcs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue1cs
dc.coverage.volume12cs
dc.date.accessioned2020-08-04T11:03:53Z
dc.date.available2020-08-04T11:03:53Z
dc.date.issued2017-02-14cs
dc.description.abstractCdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation) of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3) and normal (PNT1A) cells (detection limit of 500 pM of DNA), which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments.en
dc.formattextcs
dc.format.extent1277-1291cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Nanomedicine. 2017, vol. 12, issue 1, p. 1277-1291.en
dc.identifier.doi10.2147/IJN.S121840cs
dc.identifier.issn1178-2013cs
dc.identifier.other134303cs
dc.identifier.urihttp://hdl.handle.net/11012/193260
dc.language.isoencs
dc.publisherDove Medical Presscs
dc.relation.ispartofInternational Journal of Nanomedicinecs
dc.relation.urihttps://www.dovepress.com/using-cdteznse-coreshell-quantum-dots-to-detect-dna-and-damage-to-dna-peer-reviewed-fulltext-article-IJNcs
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1178-2013/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/cs
dc.subjectnanoparticlesen
dc.subjectnucleobasesen
dc.subjectbiosensoren
dc.subjectfluorescenceen
dc.subjectmutationen
dc.titleUsing CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNAen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-134303en
sync.item.dbtypeVAVen
sync.item.insts2021.03.13 00:56:00en
sync.item.modts2021.03.13 00:15:00en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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