CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

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dc.contributor.authorVaněčková, Terezacs
dc.contributor.authorBezděková, Jaroslavacs
dc.contributor.authorTvrdoňová, Michaelacs
dc.contributor.authorVlčnovská, Marcelacs
dc.contributor.authorNovotná, Veronikacs
dc.contributor.authorNeuman, Jancs
dc.contributor.authorŠtossová, Anetacs
dc.contributor.authorKanický, Viktorcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorVaculovič, Tomášcs
dc.coverage.issue1cs
dc.coverage.volume9cs
dc.date.accessioned2020-08-04T11:03:46Z
dc.date.available2020-08-04T11:03:46Z
dc.date.issued2019-08-14cs
dc.description.abstractFor the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.en
dc.formattextcs
dc.format.extent1-9cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2019, vol. 9, issue 1, p. 1-9.en
dc.identifier.doi10.1038/s41598-019-48290-2cs
dc.identifier.issn2045-2322cs
dc.identifier.other158577cs
dc.identifier.urihttp://hdl.handle.net/11012/180684
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttp://link.springer.com/article/10.1038/s41598-019-48290-2cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectCdS quantum dots (QDs)en
dc.subjectmolecularly imprinted polymer (MIP)en
dc.subjectlaser ablation ICP-MSen
dc.titleCdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detectionen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-158577en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:03:46en
sync.item.modts2020.08.04 12:36:01en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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