High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models

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dc.contributor.authorPinto, Raquelcs
dc.contributor.authorMatula, Jancs
dc.contributor.authorGomez Lazaro, Mariacs
dc.contributor.authorSousa, Mafaldacs
dc.contributor.authorLobo, Andreacs
dc.contributor.authorZikmund, Tomášcs
dc.contributor.authorKaiser, Jozefcs
dc.contributor.authorR. Gomes, Joocs
dc.coverage.issue1cs
dc.coverage.volume12cs
dc.date.accessioned2022-12-06T07:53:23Z
dc.date.available2022-12-06T07:53:23Z
dc.date.issued2022-10-19cs
dc.description.abstractCharacterization of brain infarct lesions in rodent models of stroke is crucial to assess stroke pathophysiology and therapy outcome. Until recently, the analysis of brain lesions was performed using two techniques: (1) histological methods, such as TTC (Triphenyltetrazolium chloride), a time-consuming and inaccurate process; or (2) MRI imaging, a faster, 3D imaging method, that comes at a high cost. In the last decade, high-resolution micro-CT for 3D sample analysis turned into a simple, fast, and cheaper solution. Here, we successfully describe the application of brain contrasting agents (Osmium tetroxide and inorganic iodine) for high-resolution micro-CT imaging for fine location and quantification of ischemic lesion and edema in mouse preclinical stroke models. We used the intraluminal transient MCAO (Middle Cerebral Artery Occlusion) mouse stroke model to identify and quantify ischemic lesion and edema, and segment core and penumbra regions at different time points after ischemia, by manual and automatic methods. In the transient-ischemic-attack (TIA) mouse model, we can quantify striatal myelinated fibers degeneration. Of note, whole brain 3D reconstructions allow brain atlas co-registration, to identify the affected brain areas, and correlate them with functional impairment. This methodology proves to be a breakthrough in the field, by providing a precise and detailed assessment of stroke outcomes in preclinical animal studies.en
dc.formattextcs
dc.format.extent1-22cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2022, vol. 12, issue 1, p. 1-22.en
dc.identifier.doi10.1038/s41598-022-21494-9cs
dc.identifier.issn2045-2322cs
dc.identifier.other179611cs
dc.identifier.urihttp://hdl.handle.net/11012/208590
dc.language.isoencs
dc.publisherNature Portfoliocs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttps://www.nature.com/articles/s41598-022-12329-8cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectmicro-CTen
dc.subjecttMCAOen
dc.subjectstrokeen
dc.subjectosmium tetroxideen
dc.subjectinorganic iodineen
dc.subjectTTCen
dc.titleHigh-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke modelsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-179611en
sync.item.dbtypeVAVen
sync.item.insts2022.12.06 08:53:23en
sync.item.modts2022.12.06 08:14:55en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé instrumentace a metody pro charakterizace materiálůcs
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