Polarity-Based Sequential Extraction as a Simple Tool to Reveal the Structural Complexity of Humic Acids

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dc.contributor.authorEnev, Vojtěchcs
dc.contributor.authorSedláček, Petrcs
dc.contributor.authorKubíková, Leonacs
dc.contributor.authorSovová, Šárkacs
dc.contributor.authorDoskočil, Leošcs
dc.contributor.authorKlučáková, Martinacs
dc.contributor.authorPekař, Miloslavcs
dc.coverage.issue3cs
dc.coverage.volume11cs
dc.date.accessioned2021-05-17T10:53:06Z
dc.date.available2021-05-17T10:53:06Z
dc.date.issued2021-03-19cs
dc.description.abstractA sequential chemical extraction with a defined series of eluotropic organic solvents with an increasing polarity (trichloromethane < ethyl acetate < acetone < acetonitrile < n-propanol < methanol) was performed on peat-bog humic acid. Six organic fractions were obtained and subjected to a physicochemical characterization utilizing methods of structural and compositional analysis. Advanced spectroscopic techniques such as Attenuated Total Reflectance (ATR-FTIR), total luminescence, and liquid-state 13C NMR spectrometry were combined with elemental analysis of the organic fractions. In total, the procedure extracted about 57% (wt.) of the initial material; the individual fractions amounted from 1.1% to 19.7%. As expected, the apolar solvents preferentially released lipid-like components, while polar solvents provided organic fractions rich in oxygen-containing polar groups with structural parameters closer to the original humic material. The fraction extracted with acetonitrile shows distinct structural features with its lower aromaticity and high content of protein-like structural motifs. The last two—alcohol extracted—fractions show the higher content of carbohydrate residues and their specific (V-type) fluorescence suggests the presence of plant pigment residues. The extraction procedure is suggested for further studies as a simple but effective way to decrease the structural complexity of a humic material enabling its detail and more conclusive compositional characterization.en
dc.formattextcs
dc.format.extent1-19cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationAgronomy. 2021, vol. 11, issue 3, p. 1-19.en
dc.identifier.doi10.3390/agronomy11030587cs
dc.identifier.issn2073-4395cs
dc.identifier.other170991cs
dc.identifier.urihttp://hdl.handle.net/11012/196739
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofAgronomycs
dc.relation.urihttps://www.mdpi.com/2073-4395/11/3/587cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4395/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjecttransitional peat-bogen
dc.subjectEutric Histosolen
dc.subjecthumic aciden
dc.subjectorganic fractionen
dc.subjectsequential chemical extractionen
dc.subjectabsorption parametersen
dc.subjectFTIRen
dc.subjectexcitation-emission matrixen
dc.subject13C NMRen
dc.subjectaromaticityen
dc.subjectmolecular weighten
dc.titlePolarity-Based Sequential Extraction as a Simple Tool to Reveal the Structural Complexity of Humic Acidsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-170991en
sync.item.dbtypeVAVen
sync.item.insts2021.05.17 12:53:06en
sync.item.modts2021.05.17 12:14:21en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav fyzikální a spotřební chemiecs
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