Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots

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dc.contributor.authorRessnerová, Alžbětacs
dc.contributor.authorNovotný, Filipcs
dc.contributor.authorMichálková, Hanacs
dc.contributor.authorPumera, Martincs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorHeger, Zbyněkcs
dc.coverage.issue8cs
dc.coverage.volume15cs
dc.date.accessioned2021-12-08T11:54:29Z
dc.date.available2021-12-08T11:54:29Z
dc.date.issued2021-08-24cs
dc.description.abstractTransfection is based on nonviral delivery of nucleic acids or proteins into cells. Viral approaches are being used; nevertheless, their translational capacity is nowadays decreasing due to persistent fear of their safety, therefore creating space for the field of nanotechnology. However, nanomedical approaches introducing static nanoparticles for the delivery of biologically active molecules are very likely to be overshadowed by the vast potential of nanorobotics. We hereby present a rapid nonviral transfection of protein into a difficult-to-transfect prostate cancer cell line facilitated by chemically powered rectangular virus-sized (68 nm x 33 nm) nanorobots. The enhanced diffusion of these biocompatible nanorobots is the key to their fast internalization into cells, happening in a matter of minutes and being up to 6-fold more efficient compared to static nanorobots in a nonfueled environment. The Au/Ag plasmonic nature of these nanorobots makes them simply traceable and allows for their detailed subcellular localization. Protein transfection mediated by such nanorobots is an important step forward, challenging the field of nanomedicine and having potential in future translational medical research.en
dc.description.embargo2022-07-21cs
dc.formattextcs
dc.format.extent12899-12910cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationACS Nano. 2021, vol. 15, issue 8, p. 12899-12910.en
dc.identifier.doi10.1021/acsnano.1c01172cs
dc.identifier.issn1936-0851cs
dc.identifier.other172541cs
dc.identifier.urihttp://hdl.handle.net/11012/203120
dc.language.isoencs
dc.publisherAmerican Chemical Societycs
dc.relation"European Union (EU)" & "Horizon 2020"
dc.relation.ispartofACS Nanocs
dc.relation.projectIdinfo:eu-repo/grantAgreement/EC/H2020/759585/EU//ToMeTuM
dc.relation.urihttps://pubs.acs.org/doi/10.1021/acsnano.1c01172cs
dc.rights(C) American Chemical Societycs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1936-0851/cs
dc.subjecttransfectionen
dc.subjectnanorobotsen
dc.subjectmetallothioneinen
dc.subjectprotein deliveryen
dc.subjectprotein transfectionen
dc.titleEfficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobotsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionacceptedVersionen
sync.item.dbidVAV-172541en
sync.item.dbtypeVAVen
sync.item.insts2022.07.21 00:55:56en
sync.item.modts2022.07.21 00:14:20en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Energie budoucnosti a inovacecs
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