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Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging

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Date
2019-01-02
Author
Jarošová, Rea
Smolíková, Vendula
Dvořák, Marek
Guráň, Roman
Do, Tomáš
Ondráčková, Petra
Zítka, Ondřej
Sládek, Zbyšek
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Abstract
In last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, in different types of tissue. Cryosections are the best suited for getting as much information as possible because the preparation of samples for MALDI MSI does not include deparaffinization and antigen-retrieval steps which are needed in case of conventional formalin-fixed and paraffin-embedded (FFPE) tissue sections. To analyse infection markers like interleukins in the tissue sections of porcine lungs affected by Actinobacillus pleuropneumoniae, it was necessary to obtain very well prepared cryosections for MALDI MSI. Therefore, this work was focused on optimization of preparation of frozen porcine lungs and its cryosections with conserved morphological structure.
Keywords
cryosectioning, lungs, pig, pleuropneumonia, mass spectrometry imaging
Persistent identifier
http://hdl.handle.net/11012/178383
Document type
Peer reviewed
Document version
Final PDF
Source
MendelNet 2018. 2019, p. 506-511.
https://mendelnet.cz/artkey/mnt-201801-0105_Preparation-of-cryosections-from-frozen-porcine-pulmonary-tissue-for-MALDI-mass-spectrometry-imaging.php?back=/magno/mnt/2018/mn1.php?secid=4
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  • Chytré nanonástroje [296]
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