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dc.contributor.authorGál, Břetislavcs
dc.contributor.authorVeselý, Miroslavcs
dc.contributor.authorČolláková, Janacs
dc.contributor.authorNekulová, Martacs
dc.contributor.authorJůzová, Veronikacs
dc.contributor.authorChmelík, Radimcs
dc.contributor.authorVeselý, Pavelcs
dc.date.accessioned2020-08-04T11:04:18Z
dc.date.available2020-08-04T11:04:18Z
dc.date.issued2017-08-28cs
dc.identifier.citationPLOS ONE. 2017, vol. 12, issue 8, p. 1-14.en
dc.identifier.issn1932-6203cs
dc.identifier.other138860cs
dc.identifier.urihttp://hdl.handle.net/11012/84151
dc.description.abstractHead and neck squamous cell carcinoma is one of the most aggressive tumours and is typically diagnosed too late. Late diagnosis requires an urgent decision on an effective therapy. An individualized test of chemosensitivity should quickly indicate the suitability of chemotherapy and radiotherapy. No ex vivo chemosensitivity assessment developed thus far has become a part of general clinical practice. Therefore, we attempted to explore the new technique of coherence-controlled holographic microscopy to investigate the motility and growth of live cells from a head and neck squamous cell carcinoma biopsy. We expected to reveal behavioural patterns characteristic for malignant cells that can be used to imrove future predictive evaluation of chemotherapy. We managed to cultivate primary SACR2 carcinoma cells from head and neck squamous cell carcinoma biopsy verified through histopathology. The cells grew as a cohesive sheet of suspected carcinoma origin, and western blots showed positivity for the tumour marker p63 confirming cancerous origin. Unlike the roundish colonies of the established FaDu carcinoma cell line, the SACR2 cells formed irregularly shaped colonies, eliciting the impression of the collective invasion of carcinoma cells. Time-lapse recordings of the cohesive sheet activity revealed the rapid migration and high plasticity of these epithelial-like cells. Individual cells frequently abandoned the swiftly migrating crowd by moving aside and crawling faster. The increasing mass of fast migrating epithelial-like cells before and after mitosis confirmed the continuation of the cell cycle. In immunofluorescence, analogously shaped cells expressed the p63 tumour marker, considered proof of their origin from a carcinoma. These behavioural traits indicate the feasible identification of carcinoma cells in culture according to the proposed concept of the carcinoma cell dynamic phenotype. If further developed, this approach could later serve in a new functional online analysis of reactions of carcinoma cells to therapy. Such efforts conform to current trends in precision medicine.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.language.isoencs
dc.publisherPLOScs
dc.relation.ispartofPLOS ONEcs
dc.relation.urihttp://europepmc.org/articles/PMC5573213?pdf=rendercs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectcarcinoma cellsen
dc.subjectcoherence-controlled holographic microscopyen
dc.titleDistinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopyen
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Experimentální biofotonikacs
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
sync.item.dbidVAV-138860en
sync.item.dbtypeVAVen
sync.item.insts2020.10.29 11:05:50en
sync.item.modts2020.09.14 12:15:06en
dc.coverage.issue8cs
dc.coverage.volume12cs
dc.identifier.doi10.1371/journal.pone.0183399cs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1932-6203/cs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen


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Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International