Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells

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dc.contributor.authorStrmiska, Vladislavcs
dc.contributor.authorBuchtelová, Hanacs
dc.contributor.authorMichálek, Petrcs
dc.contributor.authorKřížková, Soňacs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorHeger, Zbyněkcs
dc.date.accessioned2019-06-20T06:10:44Z
dc.date.available2019-06-20T06:10:44Z
dc.date.issued2019-01-02cs
dc.description.abstractIt has been shown that sarcosine supplementation stimulates proliferation and invassivenes of prostate cells. Nevertheless, the exact molecular mechanism responsible for this phenomenon is not known. In the present study we demonstrate that sarcosine increases expression of calmodulin (CaM), an important intracellular signaling molecule. Through this, sarcosine activates calmodulin-dependent protein kinases signaling. Pathway of activation CaM-dependent protein kinases can activate regulation of mitosis, proliferation, cell death, gene transcription and phosphorylation/ dephosphorlation of proteins. This is done through CaM binding of four Ca2+ ions. Interestingly, in this study, we identified decrese in free Ca2+ correlating with sarcosine-induced up-regulation of CaM. The influence of CaM to cell cycle changes was further verified using post transcritrional gene silencing using CaM-siRNA complex. Co-treatment of prostate cells with CaM-siRNA and sarcosine showed decrease in CaM-dependent kinases and cell invasiveness compared to sarcosine treatment only.en
dc.formattextcs
dc.format.extent532-536cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2018. 2019, p. 532-536.en
dc.identifier.isbn978-80-7509-597-8cs
dc.identifier.other156246cs
dc.identifier.urihttp://hdl.handle.net/11012/178388
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2018cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201801-0113_Investigating-the-interplay-between-sarcosine-and-Ca-sup-2-sup-dependent-signaling-in-prostate-cells.php?back=/magno/mnt/2018/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectsarcosineen
dc.subjectcalmodulinen
dc.subjectprostateen
dc.subjectprostate canceren
dc.subjecthuman cellsen
dc.titleInvestigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cellsen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-156246en
sync.item.dbtypeVAVen
sync.item.insts2020.04.01 11:01:29en
sync.item.modts2020.04.01 05:53:47en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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