Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay

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dc.contributor.authorMonti, Paolacs
dc.contributor.authorBrázda, Václavcs
dc.contributor.authorBohálová, Natáliacs
dc.contributor.authorPorubiaková, Otíliacs
dc.contributor.authorMenichini, Paolacs
dc.contributor.authorSpeciale, Andreacs
dc.contributor.authorBocciardi, Renatacs
dc.contributor.authorInga, Albertocs
dc.contributor.authorFronza, Gilbertocs
dc.coverage.issue2cs
dc.coverage.volume12cs
dc.date.accessioned2021-05-17T10:53:06Z
dc.date.available2021-05-17T10:53:06Z
dc.date.issued2021-02-15cs
dc.description.abstractP53, P63, and P73 proteins belong to the P53 family of transcription factors, sharing a common gene organization that, from the P1 and P2 promoters, produces two groups of mRNAs encoding proteins with different N-terminal regions; moreover, alternative splicing events at Cterminus further contribute to the generation of multiple isoforms. P53 family proteins can influence a plethora of cellular pathways mainly through the direct binding to specific DNA sequences known as response elements (REs), and the transactivation of the corresponding target genes. However, the transcriptional activation by P53 family members can be regulated at multiple levels, including the DNA topology at responsive promoters. Here, by using a yeast-based functional assay, we evaluated the influence that a G-quadruplex (G4) prone sequence adjacent to the p53 RE derived from the apoptotic PUMA target gene can exert on the transactivation potential of full-length and Nterminal truncated P53 family isoforms (wild-type and mutant). Our results show that the presence of a G4 prone sequence upstream or downstream of the P53 RE leads to significant changes in the relative activity of P53 family proteins, emphasizing the potential role of structural DNA features as modifiers of P53 family functions at target promoter sites.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationGenes. 2021, vol. 12, issue 2, p. 1-13.en
dc.identifier.doi10.3390/genes12020277cs
dc.identifier.issn2073-4425cs
dc.identifier.other169563cs
dc.identifier.urihttp://hdl.handle.net/11012/196738
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofGenescs
dc.relation.urihttps://www.mdpi.com/2073-4425/12/2/277cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4425/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectp53 familyen
dc.subjectyeasten
dc.subjectG-quadruplex(G4) prone sequenceen
dc.subjectwild-type and mutant p53/p63 proteinsen
dc.subjecttransactivation potentialen
dc.titleEvaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assayen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-169563en
sync.item.dbtypeVAVen
sync.item.insts2021.07.07 16:55:56en
sync.item.modts2021.07.07 16:14:15en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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