Mass spectrometric imaging of cysteine rich proteins in human skin

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dc.contributor.authorPompeiano Vaníčková, Luciecs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorKollar, Sandorcs
dc.contributor.authorEmri, Gabrielacs
dc.contributor.authorKřížková, Soňacs
dc.contributor.authorDo, Tomášcs
dc.contributor.authorHeger, Zbyněkcs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue1cs
dc.coverage.volume125cs
dc.date.accessioned2020-08-05T10:58:09Z
dc.date.available2020-08-05T10:58:09Z
dc.date.issued2019-03-15cs
dc.description.abstractLooking insight pathological processes, metallothioneins (MTs) are considered to be potential biomarkers for monitoring of a development of various types of diseases, such as cancer. The early identification of the MTs in biological tissues could be important tool for the estimation of appropriate clinical therapy. Therefore, here we investigated the application of matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using MT-1/2 antibody for MT detection in formalin-fixed paraffin-embedded (FFPE) biopsy specimens of human skin. Principal component analyses revealed differences in the peptide/protein profiles separating healthy skin from the carcinoma specimens. Statistically significant ion peaks at m/z 6038, 6300, 6676, and 7026 were more frequently detected in squamous cell carcinoma (SCC), basal cell carcinoma (BCC) and melanoma. Using IHC, we found that MT-1/2 was significantly higher in SCC and melanoma compared to healthy skin. Surprisingly, significantly low levels of MT-1/2 were found in BCC. On one side, the results indicate important role of MTs in melanoma occurrence and progression, as on the second side, there are hidden processes associated with MTs based on differences of the occurrence of the MS peaks, which could be associated with cycling of MTs isoforms.en
dc.formattextcs
dc.format.extent270-277cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 2019, vol. 125, issue 1, p. 270-277.en
dc.identifier.doi10.1016/j.ijbiomac.2018.11.272cs
dc.identifier.issn0141-8130cs
dc.identifier.other156230cs
dc.identifier.urihttp://hdl.handle.net/11012/194755
dc.language.isoencs
dc.publisherElseviercs
dc.relation"European Union (EU)" & "Horizon 2020"
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULEScs
dc.relation.projectIdinfo:eu-repo/grantAgreement/EC/H2020/759585/EU//ToMeTuM
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0141813018356241cs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0141-8130/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectMetallothioneinen
dc.subjectSquamous cell carcinomaen
dc.subjectMelanomaen
dc.subjectMALDI MSIen
dc.subjectImmunohistochemistryen
dc.titleMass spectrometric imaging of cysteine rich proteins in human skinen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionacceptedVersionen
sync.item.dbidVAV-156230en
sync.item.dbtypeVAVen
sync.item.insts2021.03.17 00:56:14en
sync.item.modts2021.03.17 00:15:14en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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