Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts

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dc.contributor.authorHernandez Tapia, Laura Guadalupecs
dc.contributor.authorFohlerová, Zdenkacs
dc.contributor.authorŽídek, Jancs
dc.contributor.authorAlvarez-Perez, Marco Antoniocs
dc.contributor.authorČelko, Ladislavcs
dc.contributor.authorKaiser, Jozefcs
dc.contributor.authorMontufar Jimenez, Edgar Benjamincs
dc.coverage.issue8cs
dc.coverage.volume13cs
dc.date.accessioned2020-08-18T06:57:15Z
dc.date.available2020-08-18T06:57:15Z
dc.date.issued2020-05-01cs
dc.description.abstractBiofabrication and maturation of bone constructs is a long-term task that requires a high degree of specialization. This specialization falls onto the hierarchy complexity of the bone tissue that limits the transfer of this technology to the clinic. This work studied the effects of the short-term cryopreservation on biofabricated osteoblast-containing structures, with the final aim to make them steadily available in biobanks. The biological responses studied include the osteoblast post-thawing metabolic activity and the recovery of the osteoblastic function of 3D-bioprinted osteoblastic structures and beta tricalcium phosphate (beta-TCP) scaffolds infiltrated with osteoblasts encapsulated in a hydrogel. The obtained structures were cryopreserved at -80 degrees C for 7 days using dimethyl sulfoxide (DMSO) as cryoprotectant additive. After thawing the structures were cultured up to 14 days. The results revealed fundamental biological aspects for the successful cryopreservation of osteoblast constructs. In summary, immature osteoblasts take longer to recover than mature osteoblasts. The pre-cryopreservation culture period had an important effect on the metabolic activity and function maintain, faster recovering normal values when cryopreserved after longer-term culture (7 days). The use of beta-TCP scaffolds further improved the osteoblast survival after cryopreservation, resulting in similar levels of alkaline phosphatase activity in comparison with the non-preserved structures. These results contribute to the understanding of the biology of cryopreserved osteoblast constructs, approaching biofabrication to the clinical practice.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMaterials . 2020, vol. 13, issue 8, p. 1-14.en
dc.identifier.doi10.3390/ma13081966cs
dc.identifier.issn1996-1944cs
dc.identifier.other164633cs
dc.identifier.urihttp://hdl.handle.net/11012/194788
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMaterialscs
dc.relation.urihttps://www.mdpi.com/1996-1944/13/8/1966cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1996-1944/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectbiofabricationen
dc.subjectbioprintingen
dc.subjectcryopreservationen
dc.subjectbone constructen
dc.subjectosteoblasten
dc.subjectmetabolic activityen
dc.subjectALP activityen
dc.titleEffects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblastsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-164633en
sync.item.dbtypeVAVen
sync.item.insts2020.12.16 20:54:55en
sync.item.modts2020.12.16 20:14:30en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Charakterizace materiálů a pokročilé povlaky 1-06cs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé polymerní materiály a kompozitcs
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Charakterizace materiálů a pokročilé povlaky 1-06
Chytré nanonástroje
Pokročilé polymerní materiály a kompozit