Biotechnological Production of Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate-co-3-Hydroxyvalerate) Terpolymer by Cupriavidus sp. DSM 19379

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dc.contributor.authorKučera, Dancs
dc.contributor.authorNováčková, Ivanacs
dc.contributor.authorPernicová, Ivacs
dc.contributor.authorSedláček, Petrcs
dc.contributor.authorObruča, Stanislavcs
dc.coverage.issue3cs
dc.coverage.volume6cs
dc.date.accessioned2020-08-18T10:57:45Z
dc.date.available2020-08-18T10:57:45Z
dc.date.issued2019-08-26cs
dc.description.abstractThe terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 4-hydroxybutyrate (4HB) was produced employing Cupriavidus sp. DSM 19379. Growth in the presence of -butyrolactone, -caprolactone, 1,4-butanediol, and 1,6-hexanediol resulted in the synthesis of a polymer consisting of 3HB and 4HB monomers. Single and two-stage terpolymer production strategies were utilized to incorporate the 3HV subunit into the polymer structure. At the single-stage cultivation mode, -butyrolactone or 1,4-butanediol served as the primary substrate and propionic and valeric acid as the precursor of 3HV. In the two-stage production, glycerol was used in the growth phase, and precursors for the formation of the terpolymer in combination with the nitrogen limitation in the medium were used in the second phase. The aim of this work was to maximize the Polyhydroxyalkanoates (PHA) yields with a high proportion of 3HV and 4HB using different culture strategies. The obtained polymers contained 0–29 mol% of 3HV and 16–32 mol% of 4HB. Selected polymers were subjected to a material properties analysis such as differential scanning calorimetry (DSC), thermogravimetry, and size exclusion chromatography coupled with multi angle light scattering (SEC-MALS) for determination of the molecular weight. The number of polymers in the biomass, as well as the monomer composition of the polymer were determined by gas chromatography.en
dc.formattextcs
dc.format.extent74-84cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBioengineering. 2019, vol. 6, issue 3, p. 74-84.en
dc.identifier.doi10.3390/bioengineering6030074cs
dc.identifier.issn2306-5354cs
dc.identifier.other158220cs
dc.identifier.urihttp://hdl.handle.net/11012/194789
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofBioengineeringcs
dc.relation.urihttps://www.mdpi.com/2306-5354/6/3/74cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2306-5354/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpolyhydroxyalkanoatesen
dc.subjectterpolymeren
dc.subjectP(3HB-co-3HV-co-4HB)en
dc.subjectCupriavidus malaysiensisen
dc.titleBiotechnological Production of Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate-co-3-Hydroxyvalerate) Terpolymer by Cupriavidus sp. DSM 19379en
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-158220en
sync.item.dbtypeVAVen
sync.item.insts2020.08.18 12:57:44en
sync.item.modts2020.08.18 12:15:18en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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