MALDI MSI reveals the spatial distribution of protein markers in tracheobronchial lymph nodes and lung of pigs after respiratory infection

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dc.contributor.authorDo, Tomášcs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorJarošová, Reacs
dc.contributor.authorOndráčková, Petracs
dc.contributor.authorSládek, Zbyšekcs
dc.contributor.authorFaldyna, Martincs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorZítka, Ondřejcs
dc.coverage.issue5723cs
dc.coverage.volume25cs
dc.date.accessioned2021-01-27T11:56:28Z
dc.date.available2021-01-27T11:56:28Z
dc.date.issued2020-12-30cs
dc.description.abstractRespiratory infections are a real threat for humans, and therefore the pig model is of interest for studies. As one of a case for studies, Actinobacillus pleuropneumoniae (APP) caused infections and still worries many pig breeders around the world. To better understand the influence of pathogenic effect of APP on a respiratory system-lungs and tracheobronchial lymph nodes (TBLN), we aimed to employ matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI). In this study, six pigs were intranasally infected by APP and two were used as non-infected control, and 48 cryosections have been obtained. MALDI-TOF MSI and immunohistochemistry (IHC) were used to study spatial distribution of infectious markers, especially interleukins, in cryosections of porcine tissues of lungs (necrotic area, marginal zone) and tracheobronchial lymph nodes (TBLN) from pigs infected by APP. CD163, interleukin 1 beta (IL-1 beta) and a protegrin-4 precursor were successfully detected based on their tryptic fragments. CD163 and IL-1 beta were confirmed also by IHC. The protegrin-4 precursor was identified by MALDI-TOF/TOF directly on the tissue cryosections. CD163, IL-1 beta and protegrin-4 precursor were all significantly (p < 0.001) more expressed in necrotic areas of lungs infected by APP than in marginal zone, TBLN and in control lungs.en
dc.formattextcs
dc.format.extent1-16cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMOLECULES. 2020, vol. 25, issue 5723, p. 1-16.en
dc.identifier.doi10.3390/molecules25235723cs
dc.identifier.issn1420-3049cs
dc.identifier.other168281cs
dc.identifier.urihttp://hdl.handle.net/11012/195918
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMOLECULEScs
dc.relation.urihttps://www.mdpi.com/1420-3049/25/23/5723cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1420-3049/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectCD163en
dc.subjectinterleukin 1en
dc.subjectprotegrin-4 precursoren
dc.subjectMALDI MSIen
dc.subjectpig modelen
dc.subjectlungs infectionen
dc.subjectActinobacillus pleuropneumoniaeen
dc.titleMALDI MSI reveals the spatial distribution of protein markers in tracheobronchial lymph nodes and lung of pigs after respiratory infectionen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-168281en
sync.item.dbtypeVAVen
sync.item.insts2021.01.27 12:56:27en
sync.item.modts2021.01.27 12:15:45en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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