CdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killing

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dc.contributor.authorSur, Vishma Pratapcs
dc.contributor.authorKomínková, Markétacs
dc.contributor.authorBuchtová, Žanetacs
dc.contributor.authorDoleželíková, Kristýnacs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorMoulick, Amitavacs
dc.coverage.issue10cs
dc.coverage.volume9cs
dc.date.accessioned2020-08-04T11:03:49Z
dc.date.available2020-08-04T11:03:49Z
dc.date.issued2019-10-31cs
dc.description.abstractThe physical and chemical synthesis methods of quantum dots (QDs) are generally unfavorable for biological applications. To overcome this limitation, the development of a novel "green" route to produce highly-fluorescent CdSe QDs constitutes a promising substitute approach. In the present work, CdSe QDs were biosynthesized in yeast Saccharomyces cerevisiae using a novel method, where we showed for the first time that the concentration of tryptone highly affects the synthesis process. The optimum concentration of tryptone was found to be 25 g/L for the highest yield. Different methods were used to optimize the QD extraction from yeast, and the best method was found to be by denaturation at 80 degrees C along with an ultrasound needle. Multiple physical characterizations including transmission electron microscopy (TEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDX), and spectrophotometry confirmed the optical features size and shape distribution of the QDs. We showed that the novel conjugate of the CdSe QDs and a cell-penetrating peptide (hecate) can detect bacterial cells very efficiently under a fluorescent microscope. The conjugate also showed strong antibacterial activity against vancomycin-resistant Staphylococcus aureus (VRSA), methicillin-resistant Staphylococcus aureus (MRSA), and Escherichia coli, which may help us to cope with the problem of rising antibiotic resistance.en
dc.formattextcs
dc.format.extent1-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationNanomaterials. 2019, vol. 9, issue 10, p. 1-15.en
dc.identifier.doi10.3390/nano9101463cs
dc.identifier.issn2079-4991cs
dc.identifier.other160566cs
dc.identifier.urihttp://hdl.handle.net/11012/184080
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofNanomaterialscs
dc.relation.urihttps://www.mdpi.com/2079-4991/9/10/1463cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2079-4991/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectbiosynthesisen
dc.subjecttryptoneen
dc.subjectQDsen
dc.subjectcell-penetrating peptideen
dc.subjectantibacterialen
dc.titleCdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killingen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-160566en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:03:48en
sync.item.modts2020.08.04 12:20:31en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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